Acta Chir Orthop Traumatol Cech. 2008; 75(6):443-445 | DOI: 10.55095/achot2008/082

Barvení nedekalcifikované kosti - modifikovaný postup pro lepší vizualizaci kostních náhradPůvodní práce

U. MAYR-WOHLFART1, G. RAVALLI2, K. P. GÜNTHER3, S. KESSLER4,*
1 Institute of Transfusion Medicine and Immunogenetics, Ulm, Germany
2 Orthopaedic Department, University of Ulm, Ulm, Germany
3 Orthopaedic Department, University of Dresden, Dresden, Germany
4 Orthopaedic Department, Sindelfingen District Hospital, Sindelfingen, Germany

We describe a detailed embedding procedure for large bone spicemens in methyl methacrylate and a new staining method by which thin sections (appr 100 um) of undecalcified bones with synthetic implants can be coloured. Different staining effects were obtained which greatly facilitated the evaluation of sections with bone, new forming bone and especially remnants of synthetic implants. The identification and quantification of the latter is difficult in common staining techniques.
A detailed embedding - staining - mounting procedure is proposed.

Klíčová slova: bone histology, hard tissue histology, light mircroscopy, biomaterials, synthetic implants, staining, undecalcified sections

Přijato: 7. duben 2008; Zveřejněno: 1. prosinec 2008  Zobrazit citaci

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MAYR-WOHLFART U, RAVALLI G, GÜNTHER KP, KESSLER S. Barvení nedekalcifikované kosti - modifikovaný postup pro lepší vizualizaci kostních náhrad. Acta Chir Orthop Traumatol Cech. 2008;75(6):443-445. doi: 10.55095/achot2008/082. PubMed PMID: 19150001.
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    Reference

    1. DELLING, G.: Simplified metacrylate embedding method for undecalcified bone sections. Beitr. Pathol., 145: 100-5, 1972.
    2. LACZKO, J. LEVAI, G.: A simple Differential Staining Method for Semi-thin Sections of ossifying Cartilage and Bone Tissues Embedded in Epoxy Resin. Mikroskopie, 31: 1-4, 1975.
    3. MASSON, P.: Trichrome stainings and their preliminary technique. J. Tech. Meth., 12: 75-90, 1929.
    4. ROMEIS, B.: Mikroskopische Technik, 17. Aufl., München, Wien, Baltimore, Urban u. Schwarzenberg 1989.
    5. SCHENK, R.: Zur Histologischen Verarbeitung von unentkalkten Knochen. Acta anat., 60: 3-9, 1965. Přejít k původnímu zdroji...
    6. WEIGERT, C.: Eine kleine Verbesserung der Hämatoxylin-van Gieson-Methode. Zwis Mikr., 21: 1-5, 1904.

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