Acta chirurgiae orthopaedicae
et traumatologiae Čechoslovaca

Acta Chir Orthop Traumatol Cech. 2021; 88(5):344-353 | DOI: 10.55095/achot2021/051

Contribution of miRNAs and Other Inflammatory Biomarkers to the Differentiation of Inflammatory and Non-Inflammatory Joint EffusionsOriginal papers

P. KUŠNIEROVÁ^1,*, I. BYSTROŇOVÁ^1,2,5, P. WALDER³, R. HLUBEK³, D. STEJSKAL^1,4

¹ Institute of Laboratory Medicine, Department of Clinical Biochemistry, University Hospital Ostrava

² The Department of Epidemiology and Public Health, Faculty of Medicine, University of Ostrava

³ Orthopedic Department, University Hospital Ostrava

⁴ Department of Biomedical Sciences, Faculty of Medicine, University of Ostrava

⁵ Department of Epidemiology and Public Health, Faculty of Medicine, University of Ostrava

PURPOSE OF THE STUDY:
The aim of the study was to determine miR-146a-5p, miR-223-3p and miR-23a-3p by an enzyme immunoassay in patients with inflammatory and non-inflammatory joint effusion and to verify the usefulness of these miRNAs as biomarkers of joint inflammation.

MATERIAL AND METHODS:
Synovial fluid (SF) samples were collected from 82 patients. The group consisted of 60 non-inflammatory, 11 inflammatory-non-pyogenic, 11 inflammatory-pyogenic SF. SF miRNA was isolated by RNA Isolation Kit Plasma/Serum. The concentrations of miRNA were determined by enzyme-linked immunosorbent assays (ELISA), C-reactive protein, interleukin-6 and procalcitonin on automatic analyser, presepsin on POCT system, interleukin-1 and human neutrofil defensins 1-3 by ELISA.

RESULTS:
A statistically significant negative correlation was found between miR-146-5p and miR-223-3p, WBC, IL-1β, IL-6 and CRP (P < 0.05) in all groups; a statistically significant positive correlation was found between miR-223-3p and miR-23a-3p, WBC, PMN, IL-1beta, IL-6 and HNP1-3, as well as a positive correlation of miR-23a-3p with IL-1β, IL-6 and HNP1-3. A statistically significant difference was found between miR-146a-5p, miR-223-3p and miR-23a-3p and individual SF groups, P = 0.006, P < 0.001, respectively. PMN, WBC, Il-1β, IL-6, HNP 1-3 predicted the inflammatory processes with excellent diagnostic power (AUC > 0.9). The clinical relevance expressed by effect size was the strongest in miR-223-3p, PMN, IL-1 , HNP 1-3 between non-inflammatory and inflammatory-pyogenic group.

CONCLUSIONS:
Our study quantified the SF miRNA by ELISA. We have shown that miR-146a-5p, miR-223-3p and miR-23a-3p can be an important group of biomarkers for the detection and monitoring of various pathophysiological conditions in synovial fluid, including inflammatory conditions.

Keywords: miRNA, synovial fluid, inflammatory joint disease, enzyme-linked immunosorbent assay

Published: October 15, 2021  Show citation